Potensi Whey Kefir Susu Kambing Sebagai Anti-Obesitas Melalui Penghambatan Sintesis Lipid dan Aktivitas Phosphoenolpyruvate Carboxykinase (PEPCK) pada Sel Model Adiposit 3T3-L1


  • Lilik Eka Radiati Department of Animal Product Technology, Faculty of Animals Science, Universitas Brawijaya.
  • Dian Laksamana Hati Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya
  • Sri Andarini Department of Public Health, Faculty of Medicine, Universitas Brawijaya
  • Dian Handayani Nutrition Departements, Faculty of Medicine, Universitas Brawijaya
  • Djalal Rosyidi Department of Animal Product Technology, Faculty of Animals Science, Universitas Brawijaya.




aktivitas enzim, susu ferementasi, kolesterol, trigliserida


Obesitas menjadi salah satu penyebab utama sindrom metabolik dislipidemia, yang dapat sebagai  komorbid berbagai penyakit. Penggunaan obat-obatan untuk mengurangi obesitas memiliki akibat yang merugikan, oleh karena itu dikembangkan produk komplementer dari susu fermentasi  sebagai strategi non-farmakologis untuk pengelolaan dislipidemia. Pemecahan masalah obesitas dapat dilakukan melalui pendekatan adipogenesis pada sel model adiposit 3T3-L1. Tujuan penelitian ini adalah menganalisis pemberian whey-KSK terhadap  TG (Total Trigliserida), TC  (Total Kolesterol) dan aktivitas PEPCK (Phosphoenol pyruvate Carboxykinase)  sel  adiposit 3T3-L1. Metode penelitian adalah  percobaan   pemberian dosis whey-KSK yang berbeda yaitu   P1 (25mg/ml), P2 (50 mg/ml), P3 (75 mg/ml),  P4 (100 mg/ml), dan kelompok KN  (kontrol negatif) dan KP  (kontrol positif) pada adiposit 3T3-L1, dengan empat kali ulangan. Hasil penelitian menunjukkan whey-KSK  25 – 100 µg/mL dapat menurunkan  TG sebesar 35,39 – 55,32%, menurunkan TC sebesar 30,46-62,12%, menurunkan aktivitas PEPCK sebesar 27,10-82,52% dan menurunkan aktivitas spesifik PEPCK sebesar 33,06-63,34%. Kesimpulan whey-KSK dapat menghambat adipogenesis sel adiposit 3T3-L1 dan berpotensi sebagai antiobesitas.



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