Potensi Whey Kefir Susu Kambing Sebagai Anti-Obesitas Melalui Penghambatan Sintesis Lipid dan Aktivitas Phosphoenolpyruvate Carboxykinase (PEPCK) pada Sel Model Adiposit 3T3-L1

Authors

  • Lilik Eka Radiati Department of Animal Product Technology, Faculty of Animals Science, Universitas Brawijaya.
  • Dian Laksamana Hati Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya
  • Sri Andarini Department of Public Health, Faculty of Medicine, Universitas Brawijaya
  • Dian Handayani Nutrition Departements, Faculty of Medicine, Universitas Brawijaya
  • Djalal Rosyidi Department of Animal Product Technology, Faculty of Animals Science, Universitas Brawijaya.

DOI:

https://doi.org/10.21776/ub.ijhn.2022.009.02.9

Keywords:

aktivitas enzim, susu ferementasi, kolesterol, trigliserida

Abstract

Obesitas menjadi salah satu penyebab utama sindrom metabolik dislipidemia, yang dapat sebagai  komorbid berbagai penyakit. Penggunaan obat-obatan untuk mengurangi obesitas memiliki akibat yang merugikan, oleh karena itu dikembangkan produk komplementer dari susu fermentasi  sebagai strategi non-farmakologis untuk pengelolaan dislipidemia. Pemecahan masalah obesitas dapat dilakukan melalui pendekatan adipogenesis pada sel model adiposit 3T3-L1. Tujuan penelitian ini adalah menganalisis pemberian whey-KSK terhadap  TG (Total Trigliserida), TC  (Total Kolesterol) dan aktivitas PEPCK (Phosphoenol pyruvate Carboxykinase)  sel  adiposit 3T3-L1. Metode penelitian adalah  percobaan   pemberian dosis whey-KSK yang berbeda yaitu   P1 (25mg/ml), P2 (50 mg/ml), P3 (75 mg/ml),  P4 (100 mg/ml), dan kelompok KN  (kontrol negatif) dan KP  (kontrol positif) pada adiposit 3T3-L1, dengan empat kali ulangan. Hasil penelitian menunjukkan whey-KSK  25 – 100 µg/mL dapat menurunkan  TG sebesar 35,39 – 55,32%, menurunkan TC sebesar 30,46-62,12%, menurunkan aktivitas PEPCK sebesar 27,10-82,52% dan menurunkan aktivitas spesifik PEPCK sebesar 33,06-63,34%. Kesimpulan whey-KSK dapat menghambat adipogenesis sel adiposit 3T3-L1 dan berpotensi sebagai antiobesitas.

 

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