Pengaruh Pemberian Madu Trigona Terhadap Perbaikan Tes Fungsi Hati Tikus yang Mengalami Drug-induced Liver Injury

Authors

  • Sigit Dwi Pramono Pramono Konsentrasi Studi Biokimia dan Biologi Molekuler, Program Studi Magister Ilmu Biomedik, Universitas
  • Rosdiana Natzir Departemen Biokimia, Fakultas Kedokteran, Universitas Hasanuddin Makassar
  • Syahrijuita Kadir Departemen Biokimia, Fakultas Kedokteran, Universitas Hasanuddin Makassar
  • Gita Vita Soraya Departemen Biokimia, Fakultas Kedokteran, Universitas Hasanuddin Makassar
  • Muhammad Husni Cangara Departemen Patologi Anatomi, Fakultas Kedokteran, Universitas Hasanuddin Makassar
  • Burhanuddin Bahar Departemen IKM-IKK, Fakultas Kedokteran, Universitas Hasanuddin Makassar

DOI:

https://doi.org/10.21776./ub.ijhn.2022.009.01.10

Keywords:

Acetaminophen, Drug-induced Liver Injury (DILI), Madu Trigona, Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST)

Abstract

Drug-induced Liver Injury (DILI) dapat disebabkan oleh penggunaan obat seperti acetaminophen yang mengalami metabolisme di hati. Kerusakan hati dideteksi melalui peningkatan enzim transaminase. Madu trigona mengandung flavonoid yang tinggi dan memiliki peran protektif terhadap inflamasi dan stres oksidatif. Penelitian ini bertujuan mengetahui efek pemberian madu trigona sebagai hepatoprotektor terhadap kadar enzim transaminase tikus Sprague-Dawley (SD) yang diinduksi acetaminophen. Penelitian ini menggunakan desain post-test with control group design dimana 12 ekor Rattus norvegicus galur SD dibagi dalam empat kelompok: kontrol negatif, kontrol positif, perlakuan 1 dan perlakuan 2. Semua kelompok kecuali kontrol negatif diinduksi acetaminophen selama 7 hari dilanjutkan pemberian madu kelulut pada kelompok perlakuan 1 dan 2 dengan dosis masing-masing 9,3 g/kgBB dan 18,6 g/kgBB selama 7 hari. Pengukuran ALT dan AST  pada hari ke-1, ke-8 dan ke-15 menggunakan spektrofotometri. Uji statistik menggunakan One Way Anova. Hasil penelitian didapatkan perbedaan signifikan  kadar ALT (nilai p=0,000) dan AST (nilai p=0,000) pada hari ke-15 antara kelompok yang diberikan madu trigona dengan tidak diberikan setelah diinduksi acetaminophen. Sebagai kesimpulan, madu trigona memiliki efek hepatoprotektor menurunkan kadar ALT dan AST.

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Published

2022-07-30

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